POLÍMEROS MACROPOROSOS A PARTIR DE UN MONÓMERO DENDRÍTICO. ESTUDIO DEL EFECTO MULTIVALENTE EN LA INMOVILIZACIÓN DE METALES Y SU APLICACIÓN EN LA PURIFICACIÓN DE UNA PROTEÍNA SÉRICA

SERGIO DAVID GARCÍA SCHEJTMAN; PAULA VIRGINIA SUBIRADA CALDARONE; CECILIA INÉS ALVAREZ IGARZABAL; MARIA CECILIA SANCHEZ; MARISA MARTINELLI

Cordoba

Congreso; 16º Congreso Internacional de Metalurgia y Materiales SAM-CONAMET 2016; 2016

UNC, UTN, INTI, UCC, SAM y SOCHIM

Dendritic structures are multi-branched molecules with high density of functional groups, which improve multivalent interactions.[1, 2] On the other hand, macroporous monoliths are used as base-supports in different separation techniques such as chromatography due to its high porosity, reasonably low back pressure, etc.[3] With this purpose, hyper cross-linked dendronized polymers networks as macroporous monoliths were obtained with excellent yields. These samples were synthetized using N-[Tris(hydroxymethyl)methyl]acrylamide (NAT), dendritic monomer from acrylate Behera?s amine (ABA) [4], and cross-linked with N,N′-Methylenebisacrylamide (BIS). Different reaction conditions were analyzed and the optimal conditions were used (24 h at 70°C, DMSO and PEG6000/C14H29OH as porogenic mixture). The attachment of the dendron was (analyzed) proved using IR spectroscopy and thermogravimetric analysis (TGA/DTA). Porous properties were studied by scanning electron microscopy and mercury intrusionporosimetry/nitrogen adsorption. Dendron changed chemistry properties and improved morphology and porosity. Immobilized metal ions such as Cu (II) and Zn (II) were studied and it was demonstrated that the dendritic structure was responsible for the metal coordination and that when ABA concentration increases from 20 to 30% w/w in polymer (50% of increasing), the amount of immobilized metal reaches up 100% (0,13 to 0,26 mol Cu/mol of dendron) due to multivalent effect. Finally, human alpha-2-Macroglobulin was purified using immobilized metal polymers, which showed the potential application of these supports in immobilized metal affinity chromatography (IMAC).