Molecular cloning and nucleotide sequence of the streptavidin gene

ARGARAÑA CE; KUNTZ ID; BIRKEN S; AXEL R; CANTOR CR

OXFORD UNIV PRESS

Año: 1986 vol. 14 p. 1871 - 1871

sing synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library of Streptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3´ and 5´ flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.