Microscopic and PCR-based detection of microsporidia spores in human stool samples

CRISTIAN JAVIER MENA; ANDRÉS BARNES; GONZALO CASTRO; LORENA GUASCONI; VERÓNICA LILIANA BURSTEIN; IGNACIO BECCACECE; PATRICIA CATALINA PAULIN; JOEL ARNEODO; SILVANA CARNEVALE; GERMÁN ASTUDILLO; LAURA CERVI; MARTIN GUSTAVO THEUMER; LAURA CHIAPELLO

Revista Argentina de Microbiología

Asociación Argentina de Microbiología

Año: 2020

icrosporidia are obligate intracellular fungi with a remarkable ability to infecta wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is themost frequently microsporidia reported worldwide, and mainly associated with chronic diarrhea and wasting syndrome in AIDS patients. Microscopy and PCR-based detection techniquesare effective for diagnosis and identification of species and genotypes; however, these methodsshould be standardized in each laboratory. In this study, we performed microscopy and nestedPCR techniques with PCR product sequencing to detect E. bieneusi in human stool samples.These techniques, if applied together, might prove useful for diagnosis and future epidemiological studies of intestinal microsporidiosis in Argentina