Restriction fragment Length Polymorphism of the mtDNA “A+T rich” region as a genetic marker in Aedes aegypti.

RONDAN DUEÑAS, J ; PANZETTA DE DUTARI, GM; BLANCO, A; GARDENAL, CN

ENTOMOLOGICAL SOC AMER

Año: 2002 vol. 95 p. 352 - 352

BSTRACT The usefulness of the control region of mtDNA as a tool for the study of population structure in the mosquito Aedes aegypti (L.) was analyzed. Population samples were taken from several geographic areas of Argentina; one additional sample from the city of San Juan, Puerto Rico, was included for comparison. In individual mosquitoes, the AT-rich and the COII-COIII regions were ampliÞed by polymerase chain reaction using universal insect primers. Restriction fragment-length polymorphisms were investigated from ampliÞcation products. In the AT region, three ampliÞed fragments of different total length were obtained (of 2,500, 2,300, and 2,100 bp). The enzymes Ssp I, Dra I, Pac I, and Apo I produced polymorphic patterns. Including total fragment length as a variable, eight different haplotypes were resolved for Argentinian populations, some presenting a restricted geographic distribution. The coding COII-COIII region revealed very low polymorphism. Although 11 restriction