Activation of beta-Catenin signalling increases STRAD7 gene expression in JEG-3 cells

RENA V.; ANGELETTI S.; PANZETTA DE DUTARI G.; GENTI DE RAIMONDI S

W.B. Saunders Company Ltd

Lugar: England; Año: 2009 vol. 30 p. 876 - 876

tarD7 gene encodes a protein that belongs to the StAR-related lipid transfer proteins involved in intracellular transport and metabolism of lipids. It has been previously documented that StarD7 has a wide-spread mRNA expression in trophoblastic tissues and several tumour cell lines with highest levels in both choriocarcinoma JEG-3 and JAR cells, hepatocellular carcinoma HepG2, and colorectal adenocarcinoma HT-29 cells. To understand the molecular mechanisms that regulate the expression of the human StarD7 gene, we have cloned and characterized the 5´-flanking region of the gene. Transient transfections of several 5´deleted StarD7-promoter-firefly luciferase constructs into JEG-3 cells indicated that the -312/+157 region contains the gene minimal promoter. In addition, sequence analysis of a 1.6kb gene fragment revealed the presence of a TATA-less promoter as well as multiple regulatory motifs, including one regulatory element corresponding to the T-cell factor 4 (TCF4) binding site. I