Isolation and characterization of microsatellite markers in Calomys musculinus (Muridae, Sigmodontinae, Phyllotini), the natural reservoir of Junin virus

CHIAPPERO, MARINA B; GARDENAL, CRISTINA N; PANZETTA DE DUTARI GRACIELA M

Molecular Ecology Notes

Blackwell Publishing Ltd

Año: 2005 vol. 5 p. 593 - 593

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt">Calomys musculinus,one of the most abundant rodent species in central Argentina, is the reservoir of Junin virus, the aetiological agent of Argentine hemorrhagic fever. We isolated six polymorphic microsatellite loci for microgeographical studies of population structure in this species. Amplification of these loci in 36 individuals from three natural populations revealed five to 14 alleles per locus and expected heterozygosities from 0.426 to 0.868. Crossspecies amplifications suggest that primers designed for these loci may be useful in other closely related species of the tribe Phyllotini, but not in species of other more distantly related tribes of the subfamily Sigmodontinae.