Nucleotide sequence of a pregnancy-specific beta glycoprotein gene family member

GM PANZETTA DE DUTARI; JL BOCCO; B REIMUND; A FLURY; LC PATRITO

MOLECULAR BIOLOGY REPORTS

SPRINGER

Lugar: Berlin; Año: 1992 vol. 16 p. 255 - 255

0301-4851

he pregnancy-specific b1 glycoprotein (PSG) genes encode a group of heterogeneous proteins produced in large amounts by the human syncytiotrophoblast. Their expression seems to be regulated at the transcriptional level during normal pregnancy. In the present work, we isolated from a human placental library a 17 kb genomic fragment corresponding to a member of the PSG multigene family. DNA sequence analysis of 1190 nucleotides upstream of the translational start and of the first intron, revealed the presence of several putative regulatory sequences. In a transient chloramphenicol acetyltransferase expression assay, 5′ flanking sequences within 123 nucleotides upstream to the first major transcription initiation site, functioned as a strong promoter in COS-7 cells. Meanwhile, sequences 5′ further upstream had the ability to abolish this promoter activity. The sequence analyzed did not contain any obvious TATA-like boxes or G+C-rich regions, suggesting the existence of unique