The StAR-related lipid transfer (START) domain is defined as a motif of around 200

amino acids implicated in lipid/sterol binding. In a previous study, we identified the

StarD7 transcript that encodes one of the 15 family members with START domain present

in the human genome. This transcript was found over-expressed in choriocarcinoma JEG-

3 cell line. In addition, we demonstrated that the recombinant StarD7 protein forms stable

Gibbs and Langmuir monolayers at the air-buffer interface, showing marked surface activity

and interaction with phospholipid monolayers, mainly with phosphatidylserine,

cholesterol and phosphatidylglycerol. Objectives: This study was undertaken to evaluate

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

the expression and localization of StarD7 protein in trophoblastic samples. Methods:

Western blot, immunohistochemical and immunofluorescence assays were performed

with two different antibodies obtained against over-expressed StarD7 and StarD7-C-terminal

amino acids. Furthermore, StarD7 transcript levels were determined by quantitative

real time PCR. Results: Western blot assays revealed a 34 kDa protein over-expressed in

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

normal placenta. Immunohistochemical data from early and full-term normal placenta

showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical

side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore,

an increase in StarD7 mRNA and protein expression as well as a partial subcellular

protein re-localization was observed in in vitro differentiating cytotrophoblast

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

normal placenta. Immunohistochemical data from early and full-term normal placenta

showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical

side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore,

an increase in StarD7 mRNA and protein expression as well as a partial subcellular

protein re-localization was observed in in vitro differentiating cytotrophoblast

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

normal placenta. Immunohistochemical data from early and full-term normal placenta

showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical

side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore,

an increase in StarD7 mRNA and protein expression as well as a partial subcellular

protein re-localization was observed in in vitro differentiating cytotrophoblast

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

normal placenta. Immunohistochemical data from early and full-term normal placenta

showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical

side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore,

an increase in StarD7 mRNA and protein expression as well as a partial subcellular

protein re-localization was observed in in vitro differentiating cytotrophoblast

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

normal placenta. Immunohistochemical data from early and full-term normal placenta

showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical

side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore,

an increase in StarD7 mRNA and protein expression as well as a partial subcellular

protein re-localization was observed in in vitro differentiating cytotrophoblast

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

choriocarcinoma tissue and complete hydatidiform mole compared to early and full-term

normal placenta. Immunohistochemical data from early and full-term normal placenta

showed that this protein is abundant in the syncytiotrophoblast cells, mainly at the apical

side of the syncytium, with less intensive reaction in the cytotrophoblast cells. Furthermore,

an increase in StarD7 mRNA and protein expression as well as a partial subcellular

protein re-localization was observed in in vitro differentiating cytotrophoblast

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.

cells isolated from full-term normal placenta. Conclusions: Our findings suggest that

StarD7 may play a functional role in the process of trophoblast differentiation through

phospholipids uptake and transport. Supported by CONICET, FONCyT and SECyTUNC.