d-Amino acid oxidase bio-functionalized platforms: Toward anenhanced enzymatic bio-activity

ELISA HERRERA; JAVIER VALDEZ TAUBAS; CARLA E. GIACOMELLI

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 365 p. 679 - 679

The purpose of this work is to study the adsorption process and surface bio-activity of His-taggedd-amino acid oxidase (DAAO) from Rhodotorula gracilis (His6-RgDAAO) as the first step for the devel-opment of an electrochemical bio-functionalized platform. With such a purpose this work comprises: (a)the His6-RgDAAO bio-activity in solution determined by amperometry, (b) the adsorption mechanismof His6-RgDAAO on bare gold and carboxylated modified substrates in the absence (substrate/COO−)and presence of Ni(II) (substrate/COO−+ Ni(II)) determined by reflectometry, and (c) the bio-activity ofthe His6-RgDAAO bio-functionalized platforms determined by amperometry. Comparing the adsorptionbehavior and bio-activity of His6-RgDAAO on these different solid substrates allows understanding thecontribution of the diverse interactions responsible for the platform performance. His6-RgDAAO enzy-matic performance in solution is highly improved when compared to the previously used pig kidn