Artículos
Título:
Post-translational incorporation of the antiproliferative agent azatyrosine into the C-terminus of alpha-tubulin.
Autor/es:
PURRO SA, BISIG CG, CONTIN MA, BARRA HS, ARCE CA.
Revista:
BIOCHEMICAL JOURNAL
Referencias:
Año: 2003 vol. OCT p. 121 - 121
Resumen:
etyrosination/tyrosination of tubulin is a post-translational
modification that occurs at the C-terminus of the alpha-subunit, giving
rise to microtubules rich in either tyrosinated or detyrosinated
tubulin which coexist in the cell. We hereby report that the tyrosine
analogue, azatyrosine, can be incorporated into the C-terminus of
alpha-tubulin instead of tyrosine. Azatyrosine is structurally
identical to tyrosine except that a nitrogen atom replaces carbon-2 of
the phenolic group. Azatyrosine competitively excluded incorporation of
[14C]tyrosine into tubulin of soluble brain extract. A newly developed
rabbit antibody specific to C-terminal azatyrosine was used to study
incorporation of azatyrosine in cultured cells. When added to the
culture medium (Ham's F12K), azatyrosine was incorporated into tubulin
of glioma-derived C6 cells. This incorporation was reversible, i.e.
after withdrawal of azatyrosine, tubulin lost azatyrosine and
reincorporated tyrosine. Azatyrosinat