3-Nitrotyrosine Impairs Angiogenesis of Pulmonary Artery Endothelial Cells Isolated from Developing Lungs via Uncoupling eNOS Activity

, WU TJ, BISIG CG, EIS A, PRITCHARD K AND KONDURI GG

INT PEDIATRIC RESEARCH FOUNDATION, INC

Año: 2011 vol. 69 p. 112 - 112

span class="Apple-style-span" style="WORD-SPACING: 0px; FONT: medium ´Times New Roman´; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px">Infection is known to impair the growth of developing lungs. It is known that plasma free nitrotyrosine (NT) levels can reach 150 ìM during sepsis. Free NT incorporates into microtubules and impairs cell function. We hypothesize that free NT perturbs the angiogenic activity of pulmonary artery endothelial cells (PAEC) in developing lungs. PAEC from fetal lamb lungs were incubated with NT (1-100 ìM). We examined the effects of NT on tube formation, cell