Telomere-led meiotic chromosome movements: recent update in structure and function

LEE, CY; BISIG, CG (PRIMERA AUTORIA COMPARTIDA); CONRAD, MN; DITAMO, Y; PREVIATO DE ALMEIDA, L; DRESSER, ME; PEZZA, RJ

Nucleus

Taylor and Francis Group

Año: 2020

1949-1034

elomere-led chromosome movement or Rapid Prophase Movements (RPM) occur in meiotic prophase and is a conserved process required for proper homologous chromosome interactions. This type of active movements originates in the cytoplasm and is translated to the telomeres through the nuclear membrane. We highlight our recent advances in the engine generating RPMs in S. cerevisiae, with special emphasis in exonuclear components of the machinery. We demonstrate that Mps2 protein participates as a LINC complex component; we identify Myo2 motor protein, which interacts with Mps2 connecting telomeres to the actin cytoskeleton; and show that Csm4 is a regulator of the Mps2-Myo2 interaction and function. In this article we extend our observations regarding to the functional interaction of Mps2 and Csm4. We show that the Mps2-Csm4 fusion protein complement deficient sporulation and anaphase I entrance of a csm4 deletion strain, and partially complement RPM function. Overall, our results allow the