The increase in mannose receptor recycling favors arginase induction and Trypanosoma cruzi survival in macrophages

GARRIDO, VV; DULGERIAN, LR; STEMPIN, CC; CERBÁN, FM

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES

IVYSPRING INT PUBL

Año: 2011 vol. 7 p. 1257 - 1257

1449-2288

he macrophage mannose receptor (MR) is a pattern recognition receptor of the innate immune system that binds to microbial structures bearing mannose, fucose and N-acetylglucosamine on their surface. Trypanosoma cruzi antigen cruzipain (Cz) is found in the different developmental forms of the parasite. This glycoprotein has a highly mannosylated C-terminal domain that participates in the host-antigen contact. Our group previously demonstrated that Cz-macrophage (Mo) interaction could modulate the immune response against T. cruzi through the induction of a preferential metabolic pathway. In this work, we have studied in Mo the role of MR in arginase induction and in T. cruzi survival using different MR ligands. We have showed that pre-incubation of T. cruzi infected cells with mannose-Bovine Serum Albumin (Man-BSA, MR specific ligand) biased nitric oxide (NO)/urea balance towards urea production and increased intracellular amastigotes growth. The study of intracellular signals showed th