How low does the oxygen concentration go within a sandwich-type amperometric biosensor?

MARCELO RICARDO ROMERO; ANA M. BARUZZI; FERNANDO GARAY

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2012 vol. 174 p. 279 - 279

font size="1" face="GulliverRM"> Software for the automatic non-linear least squares fit of chronoamperometric responses corresponding to sandwich-type amperometric biosensors has been developed. The so-called Simplex algorithm computes a minimum value for the difference between experimental and theoretical data. The latter consider a numerical model based on a ping-pong reaction mechanism corresponding to an oxidase enzyme that has been immobilized between diffusion membranes. The results obtained from the simulation of a first-generation lactate biosensor in presence of 0.1 mM substrate indicate that the concentration of O2 would decrease only 0.1% with regards to its bulk value. Besides, the concentration of this natural mediator would remain practically unchanged