Design and optimization of a lactate amperometric biosensor

MARCELO RICARDO ROMERO; FERNANDO GARAY; ANA M. BARUZZI

ELSEVIER SCIENCE SA

Año: 2008 vol. 131 p. 590 - 590

font size="1" face="Times-Roman"> The design and characterization of a lactate biosensor is described. The biosensor is developed through the immobilization of lactate oxidase (LOD) in an albumin and mucin composed hydrogel. The enzyme is then cross-linked with glutaraldehyde to the polymeric matrix and entrapped between two polycarbonate membranes. The hydrogen peroxide produced by the reaction of lactate and LOD is detected on a Pt electrode operated at 0.65V versus Ag|AgCl. The performance of the biosensor was ev