Mucosal immunization with polymeric antigen BLSOmp31 using alternative delivery systems against Brucella ovis in rams

DÍAZ, ALEJANDRA GRACIELA; QUINTEROS, DANIELA ALEJANDRA; PAOLICCHI, FERNANDO ALBERTO; RIVERO, MARIANA ALEJANDRA; PALMA, SANTIAGO DANIEL; PARDO, ROMINA PAOLA; CLAUSSE, MARÍA; ZYLBERMAN, VANESA; GOLDBAUM, FERNANDO ALBERTO; ESTEIN, SILVIA MARCELA

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2019

0165-2427

ubcellular vaccines against ovine contagious epididymitis due Brucella ovis can solve some shortcomings associated with the use of Brucella melitensis Rev 1. We have demonstrated that the parenteral immunization with polymeric antigen BLSOmp31 emulsified in oil adjuvant conferred significant protection against B. ovis in rams. In our previous studies, we have characterized chitosan microspheres (ChMs) and a thermoresponsive and mucoadhesive in situ gel (Poloxamer 407-Ch) as two novel formulation strategies for the delivery of BLSOmp31 in nasal as well as conjunctival mucosa. In the present work, we evaluated the immunogenicity and protection conferred by the intranasal and conjunctival immunization with these two mucosal delivery systems against B. ovis in rams. BLSOmp31-ChM administered by intranasal route and BLSOmp31-P407-Ch applied by intranasal or conjunctival routes induced systemic, local and preputial IgG and IgA antibody response. Neither formulation showed interference in th