Spherical silver nanoparticles in the detection of thermally denatured collagens

MANUEL AHUMADA; SARA MCLAUGHLIN; NATALIA L. PACIONI; EMILIO I. ALARCON

Analytical and Bioanalytical Chemistry

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2016 vol. 408 p. 1993 - 1993

1618-2642

e have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation.Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution ofpure collagen was 14.7, 8.5, and 8.6 μg mL?1 for porcine (PCOL), rat tail (RCOL), and type I human recombinant(HCOL) collagen, respectively.