KRÜPPEL-LIKE FACTOR 6 (KLF6) REQUIRES ITS AMINO TERMINAL DOMAIN TO PROMOTE VILLOUS TROPHOBLAST CELL FUSION

MIRANDA, ANDREA L; RACCA, ANA C.; KOURDOVA, LUCILLE T; ROJAS, MARIA L.; CRUZ DEL PUERTO, MARIANO; RODRIGUEZ-LOMBARDI, G; SALAS, ANDREA V; TRAVELLA, CLAUDIA; DA SILVA, ELAINE; DE SOUZA, S; FONSECA, E; MARQUES, A; BORBELY, A.; GENTI-RAIMONDI, S.; PANZETTA-DUTARI, GRACIELA M.

PLACENTA

W B SAUNDERS CO LTD

Lugar: Londres; Año: 2021

0143-4004

ntroduction: Villous cytotrophoblast (vCTB) cells fuse to generate and maintain the syncytiotrophoblast layer required for placental development and function. Krüppel-like factor 6 (KLF6) is a ubiquitous transcription factor with an N-terminal acidic transactivation domain and a C-terminal zinc finger DNA-binding domain. KLF6 is highly expressed in placenta, and it is required for proper placental development. We have demonstrated that KLF6 is necessary for cell fusion in human primary vCTBs, and in the BeWo cell line. Materials and Methods: Full length KLF6 or a mutant lacking its N-terminal domain were expressed in BeWo cells or in primary vCTB cells isolated from human term placentas. Cell fusion, gene and protein expression, and cell proliferation were analyzed. Moreover, Raman spectroscopy and atomic force microscopy (AFM) were used to identify biochemical, topography, and elasticity cellular modifications. Results: The increase in KLF6, but not the expression of its deleted muta