Impact of chlorpyrifos on human villous trophoblasts and chorionic villi

RIDANO, M.E.; RACCA, A.C.; FLORES-MARTIN, J.B.; FRETES, R.; BANDEIRA, C.L.; REYNA, L.; BEVILACQUA, E.; GENTI-RAIMONDI, S.; PANZETTA-DUTARI, G.M.

TOXICOLOGY AND APPLIED PHARMACOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2017 vol. 329 p. 26 - 26

0041-008X

lacental barrier regulates maternal-fetal interchange protecting the baby from damage caused by substances found in the uterine environment or circulating in the vascular system. Organophosphate (OP) pesticides are a paramount group of environmental pollutants used in intensive agriculture for protection against diseases and pests. While many studies have reported an increased risk of pregnancy alterations in pregnant women exposed to OPs, few have analyzed the effects caused by these pesticides in the placenta. Herein, we evaluated the effects of chlorpyrifos (CPF), one of the most widely used OP insecticides, on human placenta using in vitro and ex vivo exposure models. Villous cytotrophoblast cells isolated from normal human term placentas maintained their cell viability, differentiated into syncytiotrophoblast-like structures, and increased the expression of β-hCG, ABCG2, and P-gp in the presence of CPF at concentrations of 10 to 100μM. The same doses of CPF induced mark