Nitric oxide donors inhibit iodide transport and organification and induce morphological changes in cultured bovine thyroid cells

COSTAMAGNA MARÍA E, CABANILLAS ANA M, COLEONI ALDO H, PELLIZAS CLAUDIA G, MASINI-

THYROID

Mary Ann Liebert, Inc.

Lugar: New York, U.S.A.; Año: 1998 vol. 8 p. 1127 - 1127

1050-7256

dir> Nitric oxide (NO) has been proposed as an intracellular signal in the thyroid. The NO effect on function and morphology of bovine thyroid follicles in culture was analyzed by using the NO donors sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO). Both NO donors induced a concentration-dependent NO release measured by the nitrite accumulation in the culture medium. The SNP (10 to 500 micromol/L) treatment for 24 hours significantly inhibited the uptake, organification and transport of iodide in a concentration-dependent manner. When SNP (50 micromol/L) was withdrawn from the culture medium after 24 hours' incubation, iodide uptake and organification were partially recovered at 24 hours and reached the control value at 48 hours, indicating a reversible effect of SNP. A possible involvement of cyanide in the SNP inhibitory effect was excluded because incubation of follicles with potassium cyanide (KCN) at concentrations estimated