Nitric oxide/cGMP signaling inhibits TSH-stimulated iodide uptake and expression of Thyroid Peroxidase and Thyroglobulin mRNA in FRTL-5 thyroid cells

BAZZARA LG; VÉLEZ ML; COSTAMAGNA ME ; CABANILLAS AM; FOZZATTI L; LUCERO AM; PELLIZAS CG; MASINI REPISO AM

MARY ANN LIEBERT INC

Lugar: New York; Año: 2007 vol. 17 p. 717 - 717

font size="2" face="AdvP7C34"> OBJECTIVE: Nitric oxide (NO) induces morphological and functional alterations in primary cultured thyroid cells. The aim of this paper was to analyze the direct influence of a long-term exposition to NO on parameters of thyroid hormone biosynthesis in FRTL-5 cells. DESIGN: Cells were treated with the NO donor sodium nitroprusside (SNP) for 24-72 h. MAIN OUTCOME: SNP (50-500 micromol/L) reduced iodide uptake in a concentration-dependent manner. The inhibition of iodide uptake increased progressively with time and matched nitrite accumulation. SNP inhibited thyroperoxidase (TPO) and thyroglobulin (TG) mRNA expression in a concentration-dependent manner. SNP enhanced 3',5'-cyclic guanosine monophosphate (cGMP) production. 3',5'-cyclic adenosine phosphate (cAMP) generation was reduced by a high SNP concentration after 48 h. 8-Bromoguanosine 3',5'-cyclic monophosphate (8-Br-cG