The Transcription Factor NF-κB Mediates Thyrotropin-Stimulated Expression of Thyroid Differentiation Markers

MASINI-REPISO, ANA MARÍA

THYROID

MARY ANN LIEBERT INC

Lugar: New York; Año: 2021 vol. 31 p. 299 - 299

1050-7256

ackground: The nuclear factor kappa-light-chain-enhancer of activated B cells (NF-jB) transcription factor is a key regulator of cell survival, proliferation, and gene expression. Although activation of NF-jB signaling in hyroid follicular cells after thyrotropin (TSH) receptor (TSHR) engagement has been reported, the downstream signaling leading to NF-jB activation remains unexplored. Here, we sought to elucidate the mechanisms that regulate NF-jB signaling activation in response to TSH stimulation.Methods: Fisher rat-derived thyroid cell lines and primary cultures of NF-jB essential modulator (NEMO)-deficient mice thyrocytes were used as models. Signaling pathways leading to the activation of NF-jB were investigated by using chemical inhibitors and phospho-specific antibodies. Luciferase reporter gene assays and site-directed mutagenesis were used to monitor NF-jB-dependent gene transcriptional activity and the expression of thyroid differentiation markers was assessed by reverse tr