Activation of beta-catenin signaling increases StarD7 gene expression in JEG-3 cells

RENA VIVIANA; ANGELETTI SOFÍA; PANZETTA-DUTARI GRACIELA; GENTI DE RAIMONDI SUSANA

W.B. Saunders

Lugar: London; Año: 2009 vol. 30 p. 876 - 876

p style="TEXT-INDENT: 35.45pt; MARGIN: 0cm -3.95pt 0pt 0cm; mso-layout-grid-align: none; tab-stops: 437.45pt" class="MsoNormal">StarD7 gene encodes a protein that belongs to the StAR-related lipid transfer proteins involved in intracellular transport and metabolism of lipids. It has been previously documented that StarD7 has a wide-spread mRNA expression in trophoblastic tissues and several tumour cell lines with highest levels in both choriocarcinoma JEG-3 and JAR cells, hepatocellular carcinoma HepG2, and colorectal adenocarcinoma HT-29 cells. To understand the molecular mechanisms that regulate the expression of the human StarD7 gene, we have cloned and characterized the 5?Lflanking region of the gene. Transient transfections of several 5?Ldeleted StarD7-promoter-firefly luciferase constructs into JEG-3 cells indicated that the -312/+157 region contains the gene minimal promoter. In addition, sequence analysis of a 1.6 kb gene fragment revealed the presence of a TAT