Steroid inducible transcription of the beta-hydroxysteroid dehydrogenase gene (hsd) in Comamonas testosteroni

CABRERA JULIO E; PRUNEDA-PAZ JL; GENTI DE RAIMONDI SUSANA

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Oxford; Año: 2000 vol. 73 p. 147 - 147

he expression of the Comamonas testosteroni gene, encoding 3b/17b-hydroxysteroid dehydrogenase enzyme (3b/17b-HSD), was analyzed at the transcriptional level. Northern blot analysis detected a 1 kb transcript in bacterial cells grown in minimum media supplemented with Casamino acids and testosterone. Also this transcript was observed when cells were grown in presence of 1-dehydrotestosterone, androstenedione and 1,4-androstadien-3,17dione, but not in presence of acetate, citrate, cholic acid, cholesterol, and cortisol. In addition, this e?ect was dependent on the presence of another carbon source in the growth medium used, revealing catabolite repression.