Carbonyl reduction of insecticide of an anti-inset agent imidazole analogue of metyrapone in soil bacteria, invertebrate and vertebrate species

OPPERMANN UDO; NAGEL GERD; BELAI IVAN; BUELD JULIANE; GENTI DE RAIMONDI SUSANA; KOOLMAN JAN; NETTER KARL; MASER EDMUND

ELSEVIER IRELAND LTD

Año: 1998 vol. 114 p. 211 - 211

arbonyl reduction to the respective alcohol metabolites of the anti-insect agent imidazole analogue of metyrapone, NKI 42255 (2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1- propanone) and its parent compound metyrapone was characterized in subcellular fractions previously described bacterial and mammalian hydroxysteroid dehydrogenases:carbonyl from soil bacteria, as well as insect, invertebrate and teleost species. The enzymes involved in this metabolic step were characterized with respect to their cosubstrate specificities, inhibitor susceptibilities, and immunological crossreactivities with antibodies directed against reductases (HSD:CR). All fractions investigated rapidly reduced metyrapone, with highest specific activities found in insect, invertebrate and vertebrate fractions. Except for the insect fractions, all species examined reduced the NKI compound. Cosubstrate dependence and inhibitor specificities suggest that the enzymes described belong to the protein superfa