CHLORPYRIFOS INDUCES ENDOPLASMIC RETICULUM STRESS IN JEG-3 CELLS

REYNA LUCIANA; FLORES-MARTÍN, JÉSICA; RIDANO, MAGALÍ; PANZETA DE DUTARI GRACIELA; GENTI DE RAIMONDI SUSANA

TOXICOLOGY IN VITRO : AN INTERNATIONAL JOURNAL PUBLISHED IN ASSOCIATION WITH BIBRA.

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2017 vol. 40 p. 88 - 88

0887-2333

hlorpyrifos (CPF) is an organophosphorous pesticide widelyused in agricultural, industrial, and household applications. We havepreviously shown that JEG-3 cells are able to attenuate the oxidativestress induced by CPF through the adaptive activation of the Nrf2/AREpathway. Considering that there is a relationship between oxidativestress and endoplasmic reticulum stress (ER), herein we investigatedwhether CPF also induces ER stress in JEG-3 cells. Cells were exposed to50 μM or 100 μM CPF during 24 h in conditions where cell viability wasnot altered. Western blot and PCR assays were used to explore the proteinand mRNA levels of ER stress biomarkers, respectively. CPF induced anincrease of the typical ER stress-related proteins, such as GRP78/BiP andIRE1α, a sensor for the unfolded protein response, as well as in phospho eIF2α and XBP1 mRNA splicing. Additionally, CPF led to a decrease in p53 protein expression. The downregulation of p53 levels induced by CPF was part