Impact of chlorpyrifos on human villous trophoblasts and chorionic villi

RIDANO, M.E.; RACCA, A.C.; FLORES-MARTIN, J.B.; FRETES, R.; BANDEIRA, C.L.; REYNA, L.; BEVILACQUA, E.; GENTI-RAIMONDI, S.; PANZETTA-DUTARI, G.M.

TOXICOLOGY AND APPLIED PHARMACOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2017 vol. 329 p. 26 - 26

0041-008X

lacental barrier regulates maternal-fetal interchange protecting the baby from damage caused by substancesfound in the uterine environment or circulating in the vascular system. Organophosphate (OP) pesticides are aparamount group of environmental pollutants used in intensive agriculture for protection against diseases andpests. While many studies have reported an increased risk of pregnancy alterations in pregnant women exposedto OPs, few have analyzed the effects caused by these pesticides in the placenta.Herein,we evaluated the effects of chlorpyrifos (CPF), one of themostwidely used OP insecticides, on human placentausing in vitro and ex vivo exposure models. Villous cytotrophoblast cells isolated from normal human termplacentas maintained their cell viability, differentiated into syncytiotrophoblast-like structures, and increased theexpression of β-hCG, ABCG2, and P-gp in the presence of CPF at concentrations of 10 to 100 μM. The samedoses ofCPF induced marked changes i