Ionic complexation improves wound healing in deep second-degree burns and reduces in-vitro ciprofloxacin cytotoxicity in fibroblasts

SANCHEZ, MARÍA FLORENCIA; GUZMAN, MARÍA LAURA; FLORES-MARTÍN, JESICA; CRUZ DEL PUERTO, MARIANO; LAINO, CARLOS; SORIA, ELIO ANDRÉS; DONADIO, ANA CAROLINA; GENTI-RAIMONDI, SUSANA; OLIVERA, MARÍA EUGENIA

Scientific Reports

Nature Research

Lugar: Londres; Año: 2022 vol. 12

he development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells. In this study, antibiotic-anesthetic (AA) smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as films and hydrogels. Ionic complexation with sodium alginate and hyaluronate decreased cytotoxicity of ciprofloxacin above 70% in a primary culture of isolated fibroblasts (p < 0.05). In addition, the relative levels of the proteins involved in cell migration, integrin β1 and p-FAK, increased above 1.5 times (p < 0.05) with no significant differences in cell mobility. Evaluation of the systems in a deep second-degree burn model revealed that reepithelization rate was AA-films = AA-hydrogels > control films > no treated > reference cream (silver sulfadiazine cream). In addition, appendage conservation and complete dermis