GENTI DE RAIMONDI SUSANA DEL VALLE
Congresos y reuniones científicas
Título:
Gene expression profile in StarD7 siRNA-transfected cells
Autor/es:
FLORES-MARTÍN JÉSICA; RENA VIVIANA; PANZETTA-DUTARI GRACIELA; GENTI DE RAIMONDI SUSANA
Lugar:
San Miguel de Tucumán, Tucumán
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
StarD7 protein is a member of the StarD2 subfamily of START
domain proteins, whose function remains poorly defined. We have
recently reported that StarD7 is expressed in trophoblast cytoplasm
and is partially re-localized in in vitro differentiating
cytotrophoblast cells. Furthermore we described that ß-catenin
activates human StarD7 through Wnt/ß-catenin signalling. Herein,
we used StarD7 siRNA to block down its expression in JEG-3 cells
to pursue its function. Labeled complementary ribonucleic acid
from StarD7 siRNA- and scrambled siRNA-transfected JEG-3 cells
was hybridized to a custom Affymetrix oligonucleotide DNA
microarray. Identification of altered gene expression in both
conditions was assessed by using the Significance score (S-score)
method. The data demonstrated differential expression of 89 genes
(46 increased, 43 decreased). Among them, several Wnt signalling
pathway-associated genes were identified. The mRNA levels of six
differentially expressed genes (ß-catenin, Cnx43, iNOS, MBD2,
RII) were validated using qRT-PCR. From this genomewideSmurf2 and TGF siRNA study, novel aspects of the molecular
background of StarD7 were revealed, and thus may lead to the
identification of candidate StarD7-dependent genes probably
related to its cell function