STUDY OF HUMAN PREGNANCY-SPECIFIC GLYCOPROTEIN GENE EXPRESSION DURINGTROPHOBLAST DIFFERENTIATION

CAMOLOTTO SOLEDAD; RACCA ANA; RENA VIVIANA; PATRITO LUIS; GENTI DE RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA

Carlos Paz

Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2008

Sociedad Argentina de Investigación en Bioquímica

Human pregnancy-specific glycoproteins (PSG) gene family is composed by 11 members with above 90% sequence similarity. Their expression is an early biochemical marker of trophoblast differentiation and reduced levels of PSG proteins are associated with poor pregnancy outcome. In this report we demonstrate a differential expression pattern for individual PSG genes by qPCR in two differentiation models, choriocarcinoma JEG3 cells and primary trophoblasts. Transient transfection assays of PSG genes constructs revealed that the promoter region governs differentiationdependent activation in isolated primary trophoblasts. Meanwhile, the 5? proximal regulatory region is required to mediate transcriptional activation in JEG3 cells induced to differentiate. Epigenetic regulation of PSG genes was evaluated by treating JEG3 cells with histone deacetylases inhibitors, trichostatin A and sodium butyrate. Both reagents increase endogen PSG transcripts and protein production in a dose-dependent manner, as determined by qPCR and immunofluorescence. We conclude that PSG gene family members are transcriptionaly activated during trophoblast syncytialization reaching different expression levels among them. This activation involves regulatory elements and changes in transacting factor levels, as well as modifications in chromatin structure.