GENTI DE RAIMONDI SUSANA DEL VALLE
Congresos y reuniones científicas
Título:
COMPARATIVE DIVERSITY OF NARG GENE IN THE SUQUIA RIVER SEDIMENTS
Autor/es:
REYNA LUCIANA; WUNDERLIN DANIEL; GENTI DE RAIMONDI SUSANA
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2008
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.
Resumen:
We report the molecular characterization of narG gene in sediments
isolated from two distant sites of the Suquía River of Córdoba
Province, where different nitrate concentrations were observed.
This analysis was performed using narG RFLP, cloning and
sequencing. A total of 195 narG clones of two libraries were
screened. Among 51 RFLP patterns identified, only one was found
in both sediments. Rarefaction curves of the amino acid sequences
predicted a greater diversity from the narG genes obtained in the
sediment where the higher freshwater nitrate levels were found.
Ninety different sequences were obtained and the identities between
the deduced amino-acid sequences and those from cultivated or
environmental denitrifier bacteria deposited in the databases ranges
from 57-100%. Phylogenetic analysis grouped the 195 narG clones
in seven clusters related to the b-proteobacteria (33%), aproteobacteria
(30%), d-proteobacteria (3%), g-proteobacteria (2%)
and Thermus thermophilus (10%) narG genes. The rest of the clones
were less than 68% similar to narG sequences available in databases
suggesting that they are indigenous of the Suquía River. The results
indicate that the nitrate reducing communities present in the Suquía
River sediments are phylogenetically diverse and divergent from
previously descripted sequences.