GENTI DE RAIMONDI SUSANA DEL VALLE
Congresos y reuniones científicas
Título:
Effects of the pesticide chlorpyrifos on in vitro models of trophoblast cells
Autor/es:
RIDANO, MAGALÍ; FLORES-MARTÍN, JÉSICA; MAGNARELLI GLADIS; GENTI DE RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA
Reunión:
Congreso; IFPA Meeting 2010; 2010
Institución organizadora:
International Federation Placenta Associations
Resumen:
Environmental contaminants may disrupt trophoblast cell function and
differentiation changing the expression of specific genes. An increased risk
of spontaneous abortion, low weight offspring and intrauterine growth
restriction in pregnant women chronically exposed to organophosphate
(OP) pesticides has been reported. At present, the underlying mechanisms
involved in placental toxic effects are not fully understood. Recent studies
in other cell models indicate that OP toxic effects could be mediated
through alteration of transcription factors implicated in cell replication
and/or differentiation. Herein, we investigated whether Chlorpyrifos (Cp),
one of the most widely used OP pesticides, has any effect on genes
important for placental function. As an initial approach we employed the
choriocarcinoma-derived Jeg-3 cell line model. Cellular viability was
tested using the MTT assay and cell morphology was analyzed by
desmosome immunofluorescencent localization and nuclei staining.
Expression of glial cell missing 1 (GCM1) and krüpple-like factor 6 (KLF6)
transcription factors, as well as human chorionic gonadotropin b-subunit
(hCGb) were determined by qRT-PCR. Jeg-3 cell viability exposed to
increasing Cp concentrations (up to 100 mM) for 24 and 48 h was always
greater than 75%. No major effect was observed on cell morphology, except
for an increase in the cell number with fragmented and/or condensed
nuclei. Cp treatment barely modified KLF6 expression, while it increased
hCGb and GCMa mRNA expression. These molecules are involved in
trophoblast differentiation and have been associated with hypoxia-related
placental pathologies. Our results suggest that placental Cp toxicity may be
due in part to its effect on trophoblast differentiation. We are extending
our study to in vitro differentiating cytotrophoblasts and placental
explants to confirm this hypothesis. In sum, present data support the idea
that Cp modifies placenta gene expression and could have implications for
understanding the adverse pregnancy outcomes associated with Cp
exposure in humans.