GENTI DE RAIMONDI SUSANA DEL VALLE
Congresos y reuniones científicas
Título:
Nuclear morphology and gene expresión are altered in human trophoblast cells by organophosphates
Autor/es:
RIDANO, MAGALÍ; FLORES-MARTÍN JÉSICA; MAGNARELLI GLADIS; GENTI DE RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA
Lugar:
San Miguel de Tucumán, Tucumán
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
Gene expression and cellular differentiation are tightly controlled
by genetic and epigenetic mechanisms which may be disrupted by
toxic compounds. Epidemiological data suggest an association
between chronic organophosphate (OP) exposure of pregnant
women and an increased risk of spontaneous abortion, low weight
offspring and intrauterine growth restriction. To address the
molecular mechanism(s) underling these effects, we hypothesized
that OPs exposition alter placenta cell morphology and gene
expression. Human trophoblast derived JEG3 cell line was used as
an in vitro model to test this hypothesis. In an initial approach,
cellular viability was assayed to select chlorpyrifos, phosmet and
methylazinphos concentrations for further experiments. Cell
number with fragmented and/or condensed nuclei increased with
OP concentration (50 and 100mM) and incubation time (24 and 48h)
instead, no major effect was observed on spontaneous cell fusion.
RT-PCR analysis revealed that chlorpyrifos induced a reduction in
GCM1 and KLF6 transcription factors and an increase in StarD7
expression. In addition, immunofluorescent StarD7 protein staining
was augmented in the presence of OPs, especially in cells with
condensed nuclei. These data suggest that the OPs used in these
experiments impact on placental cell morphology and function.