GENTI DE RAIMONDI SUSANA DEL VALLE
Congresos y reuniones científicas
Título:
Histone acetylation and DNA methylation inhibitors active PSG gene expresión.
Autor/es:
CAMOLOTTO SOLEDAD; RACCA ANA; PATRITO LUIS; GENTI DE RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA
Lugar:
San Miguel de Tucumán, Tucumán
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
The human pregnancy-specific glycoprotein (PSG) gene family is
composed by 11 highly similar members whose expression
constitutes an early biochemical marker of trophoblast
differentiation. They are transcriptionally up-regulated during this
process and individual PSG members reach different expression
levels. Herein, we study the contribution of epigenetic regulation to
PSG expression through histone acetylation and DNA methylation.
For this purpose, primary cytotrophoblasts, choriocarcinoma JEG3
and cervix carcinoma HeLa cell lines were treated with histone
deacetylase inhibitors, Trichostatin A (TSA) and sodium butyrate
(NaBu), and an hypomethylating drug, 5-Aza-Deoxycytidine (aza).
Both, PSG mRNA and protein levels were dose-dependently
induced, as revealed by immunofluorescence and qRT-PCR assays.
TSA and NaBu treatment of JEG3 cells transiently transfected with
PSG3 promoter constructs enhanced reporter activity. PSG3
transcriptional activation was observed even with the shortest
promoter construct bearing a 130 bp promoter sequence overlapping
the gene transcription start site. Our results indicate that epigenetic
mechanisms are involved in PSG gene regulation, and suggest that
the proximal promoter region is recognized by transcription factors
which can mediate histone modifications.