GENTI DE RAIMONDI SUSANA DEL VALLE
Congresos y reuniones científicas
Título:
Role of Krüpel-like factor 6 in PSG gene expresión during trophoblast differentiation.
Autor/es:
RACCA ANA; CAMOLOTTO SOLEDAD; BOCCO JOSÉ LUIS; GENTI DE RAIMONDI SUSANA; PANZETTA-DUTARI GRACIELA
Lugar:
San Miguel de Tucumán, Tucumán
Reunión:
Congreso; XLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
Krüppel-like factor 6 (KLF6) is a zinc finger transcription factor
involved in differentiation, cell cycle control and proliferation in
several cell systems. It is highly expressed in human term placenta,
and its mRNA and protein levels increase during human trophoblast
cell differentiation. Along this process, KLF6 has a nuclear and
cytoplasmatic localization. Pregnancy Specific Glycoproteins
(PSGs) are early biochemical markers of trophoblast differentiation
and are required for pregnancy protection. They have been
considered as target genes of KLF6 regulation, although there is
little experimental evidence supporting such a role. Here, we
confirmed by immunohistochemistry that KLF6 is expressed on
human term placental villi. Immunofluorescence assays revealed
that KLF6 is expressed before PSG during in vitro trophoblast cell
differentiation. KLF6 over expression led to an increase in PSG
mRNA and protein levels. In addition, KLF6 transactivated PSG3
promoter activity in non differentiated cells, and this activation was
enhanced when cells were differentiated. Moreover, gel shift assays
revealed that KLF6 is linked to the PSG promoter region. Altogether
these data provide further evidences that KLF6 is a direct regulator
of PSG gene expression during placental differentiation.