Chlorpyrifos pesticide effect on villous trophoblast and explant cultures from human normal term placentas

RIDANO, MAGALÍ; RACCA ANA; FRETES RICARDO; BEVILACQUA ESTELA; GENTI DE RAIMONDI SUSANA; PANZETTA DE DUTARI GRACIELA

Foz de Iguazú

Simposio; V Latin American Symposium on Maternal Fetal Interaction & Placenta; 2013

Chlorpyrifos pesticide effect on villous trophoblast and explant cultures from human normal term placentas Ridano ME1, Racca AC1,*, Fretes R2, Bevilacqua E3, Genti-Raimondi S1, Panzetta-Dutari GM1 1CIBICI?CONICET, Facultad de Ciencias Química, Universidad Nacional Córdoba, Argentina 2Departamento de Histología y Embriología, Facultad Medicina, Universidad Nacional Córdoba, Argentina 3Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil Epidemiological data suggest that chronic exposure to Chlorpyrifos (CPF), one of the most used pesticides, leads to increased risk of pregnancy disorders. Here, we assessed the effects of CPF on villous cytotrophoblasts (CTBs) and explants from human normal-term placentas. This study was approved by the Hospital Privado Ethics Committee, Argentina. Morphology, cell viability and gene expression were analyzed in samples exposed to increasing CPF concentrations up to 100mM for up to 64h. In these conditions, CTBs differentiated to syncytium-like structures as the non-exposed control; showed higher expression of Pg-p, ABCG2 and bhCG; and did not present signs of cellular dead. However, morphological alterations and cellular dead signs were observed in placental villous explants exposed to the same CPF concentrations. These results support and extend our previous findings obtained in JEG-3 cells and suggest that CPF cytotoxicity on trophoblast may be influenced by surrounding components of the placental villi. Keywords: organophosphate pesticide, placental villi, Pg-p, ABCG2, bhCG