Assessment of Krüpel-like factor 6 function in human extravillous trophoblast cells

ANDREA MIRANDA ; DANA NEGRETTI ; LUCILE KOURDOVA; JÉSICA FLORES-MARTIN ; MARIANO CURZ DEL PUERTO ; SUSANA GENTI-RAIMONDI ; GRACIELA PANZETTA DE DUTARI

Córdoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2016

SAIB

ASSESSMENT OF KRÜPEL-LIKE FACTOR 6 FUNCTION IN HUMAN EXTRAVILLOUSTROPHOBLAST CELLSMiranda A, Negretti D, Kourdova L, Flores-Martin J, Curz Del Puerto MM, Genti-Raimondi S, Panzetta de Dutari GM.Dpto. Bioquímica Clínica, Facultad de Ciencias Químicas-UNC. CIBICI-CONICET. Argentina.E-mail: andrea_lis93@yahoo.es.Trophoblast cell differentiation into migratory extravillous trophoblasts (EVT) is critical for human placentation and embryonicdevelopment. KLF6 is a zinc-finger transcription factor that has a cellular and signal dependent tumor suppressor activity. klf6knockout mice die in-utero with a phenotype including impaired placental development, however its role in placenta is partiallyknown. We have demonstrated that KLF6 is required for proper villous trophoblast differentiation and its expression is regulated byhypoxia. In order to evaluate whether it modulates EVT migration we performed wound-healing and western blot analysis in KLF6silenced HTR8/SVneo cells (human first trimester trophoblasts with an EVT phenotype). Confluent cell monolayers, transfected with aKLF6-specific siRNA (siK) or control siRNA, were ―scratched‖ and cell migration and protein expression were assessed after 4 and24h. Cell migration tends to be higher in siK cells compared to control, although difference was statistically non-significant. Proteinexpression levels of β-catenin, connexin-43, α5 and β1 integrin subunits remained essentially unmodified. Present results suggest thatdownregulation of KLF6 does not alter the EVT differentiation markers analyzed. Further studies are required to determine thepossible role of KLF6 in EVT migration