Artículos
Título:
Biotransformation of dihydrocoumarin by Aspergillus niger.
Autor/es:
CELESTE B. AGUIRRE-PRANZONI, GABRIELA I. FURQUE, CARLOS E. ARDANAZ, ADRIANA PACCIARONI, VIRGINIA SOSA, CARLOS E. TONN, MARCELA KURINA-SANZ
Referencias:
Año: 2011 vol. 7 p. 170 - 170
Resumen:
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Aspergillus niger ATCC 11394 was used to catalyze the biotransformation of dihydrocoumarin
using two experimental conditions: growing and resting cells. Six biotransformation products (2-
7) were purified from the bio-reaction media and their structures elucidated by spectroscopic
methods and mass spectrometry. The latter technique coupled to gas chromatography was used
to analyze the time course of the biotransformations; from which results a metabolic pathway
was proposed. The use of cytochrome P450 enzyme inhibitors, as well as working under poor
oxygen conditions allowed us both to inquire about the type of enzymes involved in the process,
and to design methods to prepare only metabolites that do not c