PROTEIN ARGINYLATION IN RAT BRAIN CYTOSOL: A PROTEOMIC ANALYSIS.

M. B. DECCA, M. GALIANO, C. BOSC, D. JOB, T. RABILLOUD, J. GARIN, AND M. HALLAK

NEUROCHEMICAL RESEARCH

Año: 2006 p. 1 - 1

0364-3190

font face="Advpstib" size="2"> Abstract Arginine can be post-translationally incorporated from arginyl-tRNA into the N-terminus of soluble acceptor proteins in a reaction catalyzed by arginyl-tRNA protein transferase. In the present study, several soluble rat brain proteins that accepted arginine were identified after arginine incorporation by two dimensional electrophoresis and mass spectrometry. They were identified as: contrapsin-like protease inhibitor-3, a-1-antitrypsin, apolipoprotein E, hemopexin, calreticulin and apolipoprotein A-I. All of these proteins shared a signal sequence for the translocation of proteins across endoplasmic