Changes in the expression of cytoskeletal proteins in the CNS of Transferrin Transgenic Mice

MARTA C.B., ORTIZ E.H.., HALLAK M., BARON B., GUILLOU F., ZAKIN M.M., SOTO E.F. AND PASQUINI J.M.

Develomental Neuroscience

Año: 2002 p. 242 - 242

1421-9859

p class="abstract">Apotransferrin injected intracranially into young rats has been shown in our laboratories to induce an early differentiation of oligodendroglial cells and an increased deposition of myelin. The expression of some myelin-specific proteins such as myelin basic protein (MBP) and 2´,3´-cyclic nucleotide 3´-phosphodiesterase (CNPase) and of their mRNAs were significantly increased in these animals. Also, in the cytoskeleton obtained from isolated myelin, it was found that several microtubule associated proteins (MAPs), particularly the stable tubule only peptide (STOP) and MAP 1B, as well as actin and tubulin were markedly increased. In the present paper, we compare the changes in expression of brain and myelin cytoskeletal proteins in a newly generated transferrin transgenic mouse (Tg), overexpressing the human transferrin gene, with the results obtained in aTf-injected rats. In the myelin cytoskeletal fraction of Tg mice there was a significant increase in the expressi