Artículos
Título:
Amperometric biosensor for direct blood lactate detection
Autor/es:
MARCELO R. ROMERO, FACUNDO AHUMADA, FERNANDO GARAY AND ANA M. BARUZZI
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2010 vol. 82 p. 5568 - 5568
Resumen:
b>
An amperometric sensor for lactate quantification is
presented. The developed biosensor requires only 0.2 U
of lactate oxidase, which is immobilized in a mucin/
albumin hydrogel matrix. By protecting the platinum
surface with a Nafion membrane, typical interference
related to negatively charged species such as ascorbic acid
has been minimized to practically undetectable levels.
Electrochemical properties associated with the Nafion
membrane are assessed as a function of Nafion concentration.
In a phosphate buffer solution of pH 7.0, linear
dependence of the catalytic current upon lactate bulk
concentration was obtained between 2 and