Analysis and optimization of a hydrogel matrix for the development of a sandwich-type glucose biosensor |

COLOMBO LUCAS; ANA M. BARUZZI; FERNANDO GARAY

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2015 vol. 211

he development of a sandwich-type biosensor for glucose quantification is presented. This work is focused on the optimization of the enzymatic matrix of the biosensor. The best performance was found for an enzymatic matrix composed by 30% w/w mucin, 70% w/w albumin, 1.35 U glucose oxidase (GOX) per sensor, and glutaraldehyde diluted to 3%. The crosslinking with glutaraldehyde transforms this mixture into a hydrogel that is entrapped between two membranes of polycarbonate. The selected sandwich-type biosensor showed very good response time, sensitivity, stability, and sensor-to-sensor reproducibility.