IFNg priming is involved in the activation of arginase by oligodeoxinucleotides containing CpG motifs in murine macrophages

LISCOVSKY, M; RANOCCHIA, R; GORLINO, C; ALIGNANI, D; MORÓN, G; MALETTO, B; PISTORESI, MC

Blackwell Publishing

Lugar: Londres; Año: 2009 vol. 128 p. 159 - 159

ecognition of microbial products by macrophages (M/) stimulates an inflammatory response and plays a critical role in directing the host immune response against infection. In the present work, we showed for the first time that synthetic oligodeoxynucleotides containing unmethylated cytosine guanine motifs (CpG) are able to stimulate, in the presence of interferon-c (IFN-c), both arginase and inducible nitric oxide synthase (iNOS) in murine M/. Unexpectedly, IFN-c, a cytokine believed to be an inhibitor of arginase activity, intervened in the activation of this enzyme. A significant increase in arginase activity was observed upon a short preincubation (1 hr) with IFN-c and subsequent CpG stimulation. Therefore, a very interesting observation of this study was that the CpG-mediated arginase activity is dependent on IFN-c priming. The increase in arginase activity as a result of stimulation with CpG plus IFN-c was correlated with augmented expression of the