.- Oxidative stress, cell cycle arrest and differentiation contribute to the antiproliferative action of BSO and Calcitriol on CACO-2 cells.

LIPPINCOTT WILLIAMS & WILKINS

Lugar: Oxford; Año: 2014 vol. 25 p. 810 - 810

olon cancer prognosis and incidence are connected with vitamin D3 serum levels. The sensitivity of colon cancer cells to vitamin D could be enhanced by oxidant drugs. Objective: To evaluate the response of colon cancer cell growth to 1,25(OH)2D3 and D,L-buthionine-S,R-sulfoximine (BSO). Methods: Human colon cancer Caco-2 cells were treated with 1,25(OH)2D3, BSO, both or vehicle at different doses and times. Cell proliferation was evaluated by crystal violet staining. Cell cycle and mitochondrial membrane potential were measured by flow cytometry. Total glutathione levels, catalase, superoxide dismutase and alkaline phosphatase activities were analyzed by spectrophotometry. DNA fragmentation was evaluated by TUNEL assay. Results were statistically analysed by one way ANOVA and Bonferroni as a post-hoc test. Results: BSO and 1,25(OH)2D3 inhibited Caco-2 cell growth, effect that was higher with the combined treatment. BSO plus 1,25(OH)2D3 induced cell cycle arrest and suppress