Immunodominant proteins alpha-1 giardin and beta-giardin are expressed in both assemblages A and B of Giardia lamblia

FELIZIANI, C; MERINO, MC; RIVERO, MR; HELLMAN, H; PISTORESI, MC; RÓPOLO, A

BIOMED CENTRAL LTD

Año: 2011 p. 233 - 233

ackground: To date, eight assemblages of Giardia lamblia have been described, but only assemblages A and B are known to infect humans. Despite the fact that the genomic, biological, and clinical differences found between these two assemblages has raised the possibility that they may be considered different species, there is relatively limited information on their phenotypic differences. In the present study, we developed monoclonal antibodies against alpha-1 and beta giardin, two immunodominant proteins produced during G. lamblia infection, and studied their expression and localization in WB (assemblage A) and GS trophozoites (assemblage B). Results: The polyclonal antibodies generated against WB trophozoites, particularly those recognizing intracelular proteins as well as the proteins present at the plasma membrane (variable-specific surface proteins), showed crossreactivity with intracellular proteins in GS trophozoites. The use of monoclonal antibodies against beta giardin indica