Neutrophils exhibit differential requirements for homing molecules in their lymphatic and blood trafficking into draining lymph nodes.

GORLINO, C; RANOCCHIA, R; HARMAN, MF; GARCIA, A; CRESPO, MI; MORÓN, G; MALETTO, B; PISTORESI, MC

AMER ASSOC IMMUNOLOGISTS

Lugar: Bethesda; Año: 2014 vol. 193 p. 1966 - 1966

lthough much is described about the molecules involved in neutrophil migration from circulation into tissues, less is known about the molecular mechanisms that regulate neutrophil entry into lymph nodes (LNs) draining a local inflammatory site. Here we investigated neutrophil migration towards LNs in a context of inflammation induced by immunization of BALB/c mice with ovalbumin (OVA) emulsified in Complete Freund?s Adjuvant (CFA). We demonstrated that neutrophils can enter LNs of OVA/CFA-immunized mice not only via lymphatic vessels but also from blood, across high endothelial venules (HEVs). By adoptive transfer experiments, we showed that this influx was dependent on an inflammatory-state condition and previous neutrophil stimulation with OVA/anti-OVA immune complexes. Importantly, we have demonstrated that, in the migratory pattern to LNs, neutrophils used L-selectin and PSGL-1, Mac-1 and LFA-1 integrins and CXCR4 to get access across HEVs, while Mac-1, LFA-1 and CXCR4 were involv