Induction of NADPH oxidase activity and reactive oxygen species production by a single Trypanosoma cruzi antigen.

N GUIÑAZÚ, EA CARRERA-SILVA, MC BECERRA, A PELLEGRINI, S GEA.

ELSEVIER SCI LTD

Año: 2010 vol. 40 p. 1531 - 1531

rypanosoma cruzi is an intracellular protozoan parasite that predominantly invades mononuclear phagocytes and is able to establish a persistent infection. The production of reactive oxygen species (ROS) by phagocytes is an innate defence mechanism against microorganisms. It has been postulated that ROS such as superoxide anion (O2), hydrogen peroxide and peroxynitrite, may play a crucial role in the control of pathogen growth. However, information on parasite molecules able to trigger ROS production is scarce.In this work, we investigated whether cruzipain, an immunogenic glycoprotein from T. cruzi, was able to trigger the oxidative burst by murine cells. By employing chemiluminiscense and flow-cytometric analysis, we demonstrated that cruzipain induced ROS production in splenocytes from non-immune and cruzipain immune C57BL/6 mice and in a Raw 264.7 macrophage cell line. We also identified an O2 molecule as one of the ROS produced after antigen stimulation. Cruzipain stimulati