Immunisation with a major Trypanosoma cruzi antigen promotes pro-inflammatory

GUIÑAZÚ N, PELLEGRINI A, CARRERA-SILVA EA, AOKI MP, ET AL, GEA S

INTERNATIONAL JOURNAL FOR PARASITOLOGY

Año: 2007 p. 1243 - 1243

0020-7519

nnate and adaptive immunity collaborate in the protection of intracellularpathogens including Trypanosoma cruzi infection. However, the parasite molecules that regulate the host immune response have not been fully identified. Wepreviously demonstrated that the immunisation of C57BL/6 mice with cruzipain, an immunogenic T. cruzi glycoprotein, induced a strong specific T-cell response. In this study, we demonstrated that active immunisation with cruzipain was able tostimulate nitric oxide (NO) production by splenocytes. Immune cells also showedincreased inducible nitric oxide synthase protein and mRNA expression. Spleenadherent cells secreted high levels of IFN-gamma and IL-12. Microbicidal activityin vitro was mainly mediated by reactive nitrogen intermediaries and IFN-gamma,as demonstrated by the inhibitory effects of NO synthase inhibitor or byIFN-gamma neutralisation. Specific T-cells were essential for NO, IFN-gamma