Anticoagulant effect and action mechanism of sulphated flavonoids from Flaveria bidentis

GUGLIEMONE HA,; AGNESE AM,; NUÑEZ MONTOYA S, ; CABRERA JL.

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2002 vol. 105 p. 183 - 183

uercetin 3-acetyl-7,30,40-trisulphate (ATS) and quercetin 3,7,30,40-tetrasulphate (QTS) obtained from Flaveria bidentis (Asteraceae) wereinvestigated in vitro for anticoagulant activity. Three different concentrations of each flavonoid were assayed at different incubation times,showing at 1 mMsignificant prolongation on the activated partial thromboplastin time (APTT), less on the prothrombin time (PT), and no effecton the thrombin time (TT). In order to define the action mechanism of the anticoagulant activity, all coagulation factors were evaluated and noimportant activity decrease was observed, indicating that another mechanism is involved. Thus, thrombin inhibition mediated by antithrombinIII (ATIII) and heparin cofactor II (HCII) activation was investigated in comparison to the physiological activators, heparin and dermatansulphate (DS), respectively. As a conclusion, no activation on ATIII for neither flavonoids was observed. On the contrary,