Mass spectrometry studies of Lycopodium alkaloids lycodine-type: Sauroxine and N-Demethyl-sauroxine

DIEGO A. CIFUENTE, ; MARIANA G. VALLEJO, ; MARIA G. ORTEGA,; JOSÉ L. CABRERA, ; VÍCTOR S. MARTÍN, ; CARLOS E. TONN,; ALICIA M. AGNESE, ; CARLOS E. ARDANAZ

RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM.

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2014 vol. 28 p. 2690 - 2690

0951-4198

BSTRACT RATIONALE: Sauroxine and N-Demethyl-sauroxine are Lycopodium alkaloids lycodine-type. During the last years Lycopodium alkaloids have gained a significant interest due to their unique skeletal characteristics as well as due to their acetylcholinesterase activity. Is known that drugs that inhibit acetylcholinesterase, can be used to treat early stages of Alzheimer disease. METHODS: Sauroxine and N-Demethyl-sauroxine were isolated from aerial parts of Huperzia saururus (Lam.) Trevis. Electron ionization mass spectrometry (EI-MS) (Low Resolution) and collision-induced dissociation tandem mass spectrometry in time (CID-MS/MS) fragmentation was conducted by an ion trap, GCQ Plus mass spectrometry with MS/MS. EIhigh- resolution mass spectrometry (EI-HRMS) was performed in a magnetic sector mass spectrometer Micromass VG. RESULTS: Using GC/EI-CID-MS/MS we obtained the different fragmentation routes that connect all the ionic populations. In addition, the use of EI-HRM